"The MOXI Z automated cell counter is terrific! We have considered a number of different automated cell counters to increase efficiency and consistency in our cell culture research and are very satisfied with the MOXI Z – there is nothing not to like about it. The MOXI Z is easy to use, fits in a drawer, and has a comfortable and low volume cell injection method. We recommend the MOXI Z to anyone looking for a compact and reliable automated cell counter!"

Improve downstream results with better quality control.

95%) and repeatable particle counting and sizing for a broad range of cell types – from mammalian cells to cells as small as wine yeast and more. Since today's workflows demand accurate quality control of samples, determining cell counts precisely has a significant impact on outcomes and downstream costs.

Produce repeatable cell counts with >95% accuracy in just 8 seconds.

This ultra-small instrument uses patented microfluidic thin-film cassettes that enable automatic load and measure operation. The resulting single cell volumetric measurements are completely technique-independent. It provides the ideal alternative to the tedious manual counting associated with cytometers, or the inaccurate results associated with image-based automated cell counters (typical accuracies of 75-80%).

Assess cell culture health without reagents or dyes.

Moxi Z also automatically reports a unique cell health assessment index – MPI (Moxi Population Index) – without the need for reagents or dyes. MPI is a ratio of the cell population of interest relative to the entire particle distribution in that sample, factoring in dead cells that have shrunken or broken apart as well as other debris and contaminants in the sample. For monodisperse mammalian cultures this is closely correlated to the overall health of the sample.In the case of mixed cell populations, it's provides an assessment of the relative fraction of the largest sized population in the sample. Unlike staining-based viability methods that focus on the uptake by dead cells and do not account for contaminants and other debris, MPI provides insight on the primary population of interest (based on size) and its relationship to ALL other particles in the sample.

Measure the smallest cells at very high concentrations.

Using the new Type S cassette, you can now accurately measure the smallest cells of any automated cell counter (down to 3um average diameter) at concentrations of up to 2.5e+6/ml. This means the new Type S cassette can measure Yeast (including wine yeast) and non-spherical particles down to 14 fL in volume.

Incredible performance in a small package.

? More accurate and repeatable than any other automated cell counter
? Results in just 8 seconds
? Ultra-small and ultra-easy to use (no manual counting, no focusing of any kind)
? Complete, high-resolution cell size/count histogram
? Handles "de-aggregation" and coincidence events
? Post-processing/analysis of count distributions with user-adjustable regions/gates
? Assess cell health (MPI) without the need for reagents like Trypan blue
? Complete data analysis and data portability (connectivity to PC, and MoxiChart software package)
? No maintenance required

Cassettes

Higher Performance & Simplicity in One Disposable Cassette.

Moxi Z's unique, patent-protected thin-film cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each inpidual cell in the sample. Impedance-based cell counting of inpidual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.

No system contamination and integrated pre-filter for clogging prevention.

Moxi cassettes are designed with a unique "cell sieve" to minimize cell clumping and clogging. In addition, Moxi cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.

Used cassette indicators.

Each Moxi cassette offers two inpidual tests with an easy-to-read visual indicator marking "used" tests. Moxi Z will also alert the user if a used cassette is mistakenly inserted in the unit.

Multiple cassette options.

Users can choose from Moxi Type M or Moxi Type S cassettes depending on cell specifications. See www.orflo.com > MOXI Z > APPLICATIONS for a list of cell types and corresponding cassette types.

Type M Cassette

The Type M cassette is ideal for accurately measuring mammalian cells, or other particles 4-25 microns in average diameter. Since the technology is based on a volumetric measurement, non-spherical particles (34-8,180 fL) can also be measured accurately.

Specifications
Dynamic range:
Average cell dia:
Average volume:
Sample concentration:
Measurement time:

2 - 34 microns
4 - 25 microns
34 - 8,180 fL
3,000 – 500,000 cells/mL
8 seconds

Type S Cassette NEW!

The Type S cassette can accurately measure the smallest particles at concentrations of up to 2,500,000/ml.

The Type S cassette is ideal for accurately measuring most yeast (including wine yeast), or other particles as small as 3 microns and up to 20 microns in average diameter. This includes some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accurately.

Samples run with the Type S cassette can be as concentrated as 2,500,000 cells/mL, therefore minimizing or in some cases eliminating the need for dilutions.

Specifications
Dynamic range:
Average cell dia:
Average volume:
Sample concentration:
Measurement time:

2 - 26 microns
3 - 20 microns
14 - 4,200 fL
3,000 – 2,500,000 cells/mL
15 seconds

Higher Performance & Simplicity in One Disposable Cassette.

Moxi Z's unique, patent-protected thin-film cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each inpidual cell in the sample. Impedance-based cell counting of inpidual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.

No system contamination and integrated pre-filter for clogging prevention.

Moxi cassettes are designed with a unique "cell sieve" to minimize cell clumping and clogging. In addition, Moxi cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.

Used cassette indicators.

Each Moxi cassette offers two inpidual tests with an easy-to-read visual indicator marking "used" tests. Moxi Z will also alert the user if a used cassette is mistakenly inserted in the unit.

Multiple cassette options.

Users can choose from Moxi Type M or Moxi Type S cassettes depending on cell specifications. See www.orflo.com > MOXI Z > APPLICATIONS for a list of cell types and corresponding cassette types.

Type M Cassette

The Type M cassette is ideal for accurately measuring mammalian cells, or other particles 4-25 microns in average diameter. Since the technology is based on a volumetric measurement, non-spherical particles (34-8,180 fL) can also be measured accurately.

Specifications
Dynamic range:
Average cell dia:
Average volume:
Sample concentration:
Measurement time:

2 - 34 microns
4 - 25 microns
34 - 8,180 fL
3,000 – 500,000 cells/mL
8 seconds

Type S Cassette NEW!

The Type S cassette can accurately measure the smallest particles at concentrations of up to 2,500,000/ml.

The Type S cassette is ideal for accurately measuring most yeast (including wine yeast), or other particles as small as 3 microns and up to 20 microns in average diameter. This includes some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accurately.

Samples run with the Type S cassette can be as concentrated as 2,500,000 cells/mL, therefore minimizing or in some cases eliminating the need for dilutions.

Specifications
Dynamic range:
Average cell dia:
Average volume:
Sample concentration:
Measurement time:

2 - 26 microns
3 - 20 microns
14 - 4,200 fL
3,000 – 2,500,000 cells/mL
15 seconds

Count Precision and Accuracy Across a Broad Dynamic Range

With its broad dynamic cell size range and patented technology, Moxi Z provides highly accurate cell counts for mammalian cells, yeast, algae, and other particles, without the risk of "missing" counts. In a comparison with a gold-standard counting method, as shown here, Moxi Z is shown to have equivalent results to the gold standard. As the data demonstrates, Moxi Z achieves this performance over a broad range of concentrations and particle types. Furthermore, because the Moxi Z makes a volumetric or 3-D measurement of cell size, it provides particle sizing information that is far more precise and accurate than that provided by the 2-D image-based systems.

Count Accuracy – Low Errors and Coefficients of Variation

Over the past 50 years, the Coulter technique of electronic cell counting has universally regarded
as the gold-standard in cell counting technology due to its high level of precision. Moxi Z uses
the same underlying technique to achieve, as this data shows, the same degree of accuracy (<5%
error vs the high-end Coulter System). It accomplishes this with a precision that also matches
the significantly more expensive Coulter systems (Moxi Z CV: 4% vs Z2 CV: 3%). As is shown in this
chart, this low error and variability is far superior to that of imaging-based techniques.

Count Speed

With an average test time of a mere eight seconds, the Moxi Z yields the fastest cell counts of any available technique.

Reagent-Free Culture Health Assessment (MPI)

The Moxi Z leverages the curve fitting approach with a proprietary software algorithm to generate the MPI culture health assessment value. The MPI is generated by analyzing the culture/particle size distribution to identify the relative contributions of particle debris (created as cells breakapart and through possible microbial contributions), shrunken necrotic cells, and the curve-fit monodisperse cell population of interest. Images shown here illustrate these changes in the culture size distribution as a function of the MPI. The MPI value thereby reflects ratiometric measure of the monodisperse population counts with respect to the overall particle population profile. Consequently, the MPI provides a valuable alternative view into the health of a cell culture than traditional staining approaches, particularly with respect to the debris and microbial contaminants. In this regard, the MPI provides a rapid, general assessment of cell culture health with every count measurement without the need for reagents.