Microfluidic SP1.2 Platform
?? Microscope Axiovert 40 CFL :
Contrast option: Brightfield, Phase, Varel, Fluorescence (TRITC, FITC, etc).
Shutters: Automated Brightfield and Fluorescence.
Objectives: 10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat, 40x LD A-Plan .
Eyepieces: E-PL 10x/20 Br., 10x/20 Br. foc.
Stage: Vena8 biochip compatible stage holder, Eppendorf tubes holder.
?? Mirus 1.0 Nanopump
Specifications: Shear Stress Range of 0.05–20 dyne/cm2, Volumetric Flow Rates 100nL–20μL/minute (100μL
syringe), Sample Volume Increments Freely adjustable
Linear Velocity Range of 10 μm/s to 10 cm/s; Flow Direction Reversible; Sample Volume Aspiration
Accuracy ±1% ; Shear Stress Accuracy ±0.5%; Sample Volume Aspiration Precision < 1%.
Multichannel: 16-Way manifold allowing inpidual addressing microchannels of Vena8 biochip.
?? Motorized stage with Z-focus module.
Specifications: Marzhauser IM series stage, Travel 120mm x100 mm, Repeatability <1um, Precision ±3 μm,
Maximum Travel speed 180 mm/s.
?? Microscope Cage incubator:
Capabilities: Temperature control ± 0.10C, CO2 and humidity module.
Options: Temperature monitoring software module, black panels for use in fluorescent microscopy.
?? Deltapix DP 450 USB 2.0 camera
Specifications: 2 Mega Pixels (1,616 x 1,216); Color CDD; 1/1.8" format; Pixel 4.4 μm x 4.4 μm.
Resolution: 12 FPS @ 1,616 x 1,216 pixels; 14 FPS @ 1,280 x 1,024 pixels; 18 FPS @ 1,024 x 768 pixels; 22
FPS @ 800 x 600 pixels; 26 FPS @ 640 x 480 pixels. Dynamic Range > 66 dB.
?? Dell Optiplex 745 PC computer
Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO; Objective Imaging stage
control hardware; 19” LCD monitor. Windows XP Professional SP2; DeltaPix Viewer Software;
VenaFluxAssay and DucoCell software preinstalled.
?? VenaFluxAssay Software.
Capabilities: Execution of preset protocols, simultaneous control of pumping, scanning and image
acquisitions, sample transfer and protocol flow. Intuitive stepwise protocol interface with steps hierarchy
and status control. Office2007 GUI interface, preset and user-designed protocols.
?? DucoCell software
Specifications: Automatic Counting and Detection; Morphological parameters analyzed automatically
(Area, diameter, form-factor, ellipticity, eccentricity, orientation, perimeter, asymmetry, cell centroid
coordinates, elliptical axis); Cell sorting and filtering; Ability to analyze sub-populations based on
inclusion/exclusion of morphological parameters. Data may be exported to Excel spreadsheet
incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress). File Formats analyzed
*.jpg, *.jpeg, *.tif, *.tiff, *.bmp, *.gif, *.png.
?? Vena8 biochips pack of 10
Specifications: Minimum Sample Volume 3 μL; Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2, steps of 0.05dyne/cm2. Number of channels per biochip is 8; Volume of each channel is 0.8 μL. Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).
